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Commit 28c9a49f authored by Christopher Schmied's avatar Christopher Schmied
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Added a test .yaml for Single Channel data

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common: {
# directory that contains the bean shell scripts
bsh_directory: "/projects/pilot_spim/Christopher/snakemake-workflows/spim_registration/timelapse/",
directory_cuda: "/sw/users/schmied/packages/2015-06-08_Fiji.app.cuda/lib/",
fiji-app: "/sw/users/schmied/packages/2015-06-08_Fiji.app.cuda/ImageJ-linux64",
fiji-prefix: "/sw/bin/xvfb-run -a",
first_xml_filename: "test_unicore",
hdf5_xml_filename: '"hdf5_test_unicore"',
merged_xml: "hdf5_test_unicore_merge",
ntimepoints: 5,
angles: "0,72,144,216,288",
channels: "green",
illumination: "0",
pixel_distance_x: '0.28590106964',
pixel_distance_y: '0.28590106964',
pixel_distance_z: '1.50000',
pixel_unit: "um",
# transformation_switch: "timelapse_dublicate",
transformation_switch: "timelapse",
# fusion_switch: "deconvolution"
fusion_switch: "fusion"
}
define_xml_czi: {
first_czi: "2015-04-11_LZ2_Stock68_3.czi",
rotation_around: "X-Axis",
bsh_file: "define_czi.bsh"
}
define_xml_tif: {
image_file_pattern: 'img_TL{{t}}_Angle{{a}}.tif',
imglib_container: '"ArrayImg (faster)"',
multiple_angles: '"YES (one file per angle)"',
multiple_channels: '"NO (one channel)"',
multiple_illumination_directions: '"NO (one illumination direction)"',
multiple_timepoints: '"YES (one file per time-point)"',
type_of_dataset: '"Image Stacks (ImageJ Opener)"',
bsh_file: "define_tif_zip.bsh"
}
resave_hdf5: {
hdf5_chunk_sizes: '"{{ {{32,32,4}}, {{32,32,4}}, {{16,16,16}}, {{16,16,16}} }}"',
resave_angle: '"All angles"',
resave_channel: '"All channels"',
resave_illumination: '"All illuminations"',
resave_timepoint: '"All Timepoints"',
setups_per_partition: '0',
subsampling_factors: '"{{ {{1,1,1}}, {{2,2,1}}, {{4,4,1}}, {{8,8,1}} }}"',
timepoints_per_partition: '1',
bsh_file: "export.bsh"
}
registration: {
# "Single Channel" is not a valid choice for "Process_channel"
reg_process_channel: '"All channels"',
reg_processing_channel: '"green"',
reg_interest_points_channel: '"beads"',
reg_radius_1: '2',
reg_radius_2: '3',
reg_threshold: '0.005',
initial_sigma: '1.8',
threshold_gaussian: '0.0080',
type_of_detection: '"Difference-of-Mean (Integral image based)"',
label_interest_points: '"beads"',
reg_process_timepoint: '"Single Timepoint (Select from List)"',
reg_process_angle: '"All angles"',
reg_process_illumination: '"All illuminations"',
subpixel_localization: '"3-dimensional quadratic fit"',
detection_min_max: "find_maxima",
type_of_registration: '"Register timepoints individually"',
algorithm: '"Fast 3d geometric hashing (rotation invariant)"',
transformation_model: "Affine",
allowed_error_for_ransac: '5',
significance: '10',
fix_tiles: '"Fix first tile"',
map_back_tiles: '"Map back to first tile using rigid model"',
model_to_regularize_with: "Rigid",
lambda: '0.10',
imglib_container: '"ArrayImg (faster)"',
bsh_file: "registration.bsh"
}
xml_merge: {
bsh_file: "xml_merge.bsh"
}
timelapse: {
reference_timepoint: '0',
type_of_registration_timelapse: '"Match against one reference timepoint (no global optimization)"',
timelapse_process_timepoints: '"All Timepoints"',
bsh_file: "timelapse_registration.bsh"
}
Dublicate_transformations: {
source_dublication: "red",
target_dublication: "green",
duplicate_which_transformations: '"Replace all transformations"',
bsh_file: "Dublicate_transformations.bsh"
}
fusion: {
bsh_file: "fusion.bsh",
downsample: '4',
fused_image: '"Append to current XML Project"',
imglib2_container_fusion: '"ArrayImg"',
imglib2_data_container: '"ArrayImg (faster)"',
interpolation: '"Linear Interpolation"',
minimal_x: '190',
minimal_y: '-16',
minimal_z: '-348',
maximal_x: '1019',
maximal_y: '1941',
maximal_z: '486',
pixel_type: '"16-bit unsigned integer"',
process_angle: '"All angles"',
process_channel: '"All channels"',
process_illumination: '"All illuminations"',
process_timepoint: '"Single Timepoint (Select from List)"',
process_views_in_paralell: '"All"',
xml_output: '"Save every XML with user-provided unique id"'
}
external_transform: {
# channel setting: '"all_channels"'
channel_setting: '"green"',
transform_timepoint: '"All Timepoints"',
transform_angle: '"All angles"',
transform_channel: '"All channels"',
# illumination setting only one illumination side
transform_illumination: '"All illuminations"',
apply_transformation: '"Current view transformations (appends to current transforms)"',
define_mode_transform: '"Matrix"',
matrix_transform: '"0.5, 0.0, 0.0, 0.0, 0.0, 0.5, 0.0, 0.0, 0.0, 0.0, 0.5, 0.0"',
transformation: '"Rigid"',
bsh_file: "transform.bsh"
}
deconvolution: {
iterations: '1',
minimal_x_deco: '190',
minimal_y_deco: '-16',
minimal_z_deco: '-348',
maximal_x_deco: '1019',
maximal_y_deco: '1941',
maximal_z_deco: '486',
detections_to_extract_psf_for_channel: '"beads"',
process_timepoint: '"Single Timepoint (Select from List)"',
process_angle: '"All angles"',
process_channel: '"All channels"',
process_illumination: '"All illuminations"',
Tikhonov_parameter: '0.0006',
compute: '"in 512x512x512 blocks"',
compute_on: '"GPU (Nvidia CUDA via JNA)"',
imglib2_container: '"ArrayImg"',
osem_acceleration: '"1 (balanced)"',
psf_estimation: '"Extract from beads"',
psf_size_x: '19',
psf_size_y: '19',
psf_size_z: '25',
type_of_iteration: '"Efficient Bayesian - Optimization I (fast, precise)"',
bsh_file: "deconvolution_GPU.bsh"
}
hdf5_output: {
output_image_file_pattern: 'TP{{t}}_Chgreen_Ill0_Ang0,72,144,216,288.tif',
output_xml: '"fused_Single_Channel"',
output_hdf5_xml: '"hdf5_fused_Single_Channel"',
output_multiple_channels: '"NO (one channel)"',
output_timepoints: '0-4',
output_pixel_distance_x: 0.5718,
output_pixel_distance_y: 0.5718,
output_pixel_distance_z: 0.5718,
output_pixel_unit: 'um',
output_channels: "green",
output_data_type: "32Bit",
convert_32bit: '"[Use min/max of first image (might saturate intenities over time)]"',
output_type_of_dataset: '"Image Stacks (ImageJ Opener)"',
output_multiple_timepoints: '"YES (one file per time-point)"',
output_multiple_angles: '"NO (one angle)"',
output_illumination_directions: '"NO (one illumination direction)"',
output_imglib_container: '"ArrayImg (faster)"',
subsampling_output: '"{{ {{1,1,1}}, {{2,2,2}}, {{4,4,4}}, {{8,8,8}} }}"',
chunk_sizes_output: '"{{ {{16,16,16}}, {{16,16,16}}, {{16,16,16}}, {{16,16,16}} }}"',
# subsampling_output: '"{{ {{1,1,1}}, {{2,2,2}} }}"',
# chunk_sizes_output: '"{{ {{16,16,16}}, {{16,16,16}} }}"',
bsh_file_define: "define_output.bsh",
bsh_file_hdf5: "export_output.bsh"
}
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