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FIJI
snakemake-workflows
Commits
28c9a49f
Commit
28c9a49f
authored
9 years ago
by
Christopher Schmied
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Added a test .yaml for Single Channel data
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spim_registration/timelapse/SingleChannel.yaml
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spim_registration/timelapse/SingleChannel.yaml
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28c9a49f
common
:
{
# directory that contains the bean shell scripts
bsh_directory
:
"
/projects/pilot_spim/Christopher/snakemake-workflows/spim_registration/timelapse/"
,
directory_cuda
:
"
/sw/users/schmied/packages/2015-06-08_Fiji.app.cuda/lib/"
,
fiji-app
:
"
/sw/users/schmied/packages/2015-06-08_Fiji.app.cuda/ImageJ-linux64"
,
fiji-prefix
:
"
/sw/bin/xvfb-run
-a"
,
first_xml_filename
:
"
test_unicore"
,
hdf5_xml_filename
:
'
"hdf5_test_unicore"'
,
merged_xml
:
"
hdf5_test_unicore_merge"
,
ntimepoints
:
5
,
angles
:
"
0,72,144,216,288"
,
channels
:
"
green"
,
illumination
:
"
0"
,
pixel_distance_x
:
'
0.28590106964'
,
pixel_distance_y
:
'
0.28590106964'
,
pixel_distance_z
:
'
1.50000'
,
pixel_unit
:
"
um"
,
# transformation_switch: "timelapse_dublicate",
transformation_switch
:
"
timelapse"
,
# fusion_switch: "deconvolution"
fusion_switch
:
"
fusion"
}
define_xml_czi
:
{
first_czi
:
"
2015-04-11_LZ2_Stock68_3.czi"
,
rotation_around
:
"
X-Axis"
,
bsh_file
:
"
define_czi.bsh"
}
define_xml_tif
:
{
image_file_pattern
:
'
img_TL{{t}}_Angle{{a}}.tif'
,
imglib_container
:
'
"ArrayImg
(faster)"'
,
multiple_angles
:
'
"YES
(one
file
per
angle)"'
,
multiple_channels
:
'
"NO
(one
channel)"'
,
multiple_illumination_directions
:
'
"NO
(one
illumination
direction)"'
,
multiple_timepoints
:
'
"YES
(one
file
per
time-point)"'
,
type_of_dataset
:
'
"Image
Stacks
(ImageJ
Opener)"'
,
bsh_file
:
"
define_tif_zip.bsh"
}
resave_hdf5
:
{
hdf5_chunk_sizes
:
'
"{{
{{32,32,4}},
{{32,32,4}},
{{16,16,16}},
{{16,16,16}}
}}"'
,
resave_angle
:
'
"All
angles"'
,
resave_channel
:
'
"All
channels"'
,
resave_illumination
:
'
"All
illuminations"'
,
resave_timepoint
:
'
"All
Timepoints"'
,
setups_per_partition
:
'
0'
,
subsampling_factors
:
'
"{{
{{1,1,1}},
{{2,2,1}},
{{4,4,1}},
{{8,8,1}}
}}"'
,
timepoints_per_partition
:
'
1'
,
bsh_file
:
"
export.bsh"
}
registration
:
{
# "Single Channel" is not a valid choice for "Process_channel"
reg_process_channel
:
'
"All
channels"'
,
reg_processing_channel
:
'
"green"'
,
reg_interest_points_channel
:
'
"beads"'
,
reg_radius_1
:
'
2'
,
reg_radius_2
:
'
3'
,
reg_threshold
:
'
0.005'
,
initial_sigma
:
'
1.8'
,
threshold_gaussian
:
'
0.0080'
,
type_of_detection
:
'
"Difference-of-Mean
(Integral
image
based)"'
,
label_interest_points
:
'
"beads"'
,
reg_process_timepoint
:
'
"Single
Timepoint
(Select
from
List)"'
,
reg_process_angle
:
'
"All
angles"'
,
reg_process_illumination
:
'
"All
illuminations"'
,
subpixel_localization
:
'
"3-dimensional
quadratic
fit"'
,
detection_min_max
:
"
find_maxima"
,
type_of_registration
:
'
"Register
timepoints
individually"'
,
algorithm
:
'
"Fast
3d
geometric
hashing
(rotation
invariant)"'
,
transformation_model
:
"
Affine"
,
allowed_error_for_ransac
:
'
5'
,
significance
:
'
10'
,
fix_tiles
:
'
"Fix
first
tile"'
,
map_back_tiles
:
'
"Map
back
to
first
tile
using
rigid
model"'
,
model_to_regularize_with
:
"
Rigid"
,
lambda
:
'
0.10'
,
imglib_container
:
'
"ArrayImg
(faster)"'
,
bsh_file
:
"
registration.bsh"
}
xml_merge
:
{
bsh_file
:
"
xml_merge.bsh"
}
timelapse
:
{
reference_timepoint
:
'
0'
,
type_of_registration_timelapse
:
'
"Match
against
one
reference
timepoint
(no
global
optimization)"'
,
timelapse_process_timepoints
:
'
"All
Timepoints"'
,
bsh_file
:
"
timelapse_registration.bsh"
}
Dublicate_transformations
:
{
source_dublication
:
"
red"
,
target_dublication
:
"
green"
,
duplicate_which_transformations
:
'
"Replace
all
transformations"'
,
bsh_file
:
"
Dublicate_transformations.bsh"
}
fusion
:
{
bsh_file
:
"
fusion.bsh"
,
downsample
:
'
4'
,
fused_image
:
'
"Append
to
current
XML
Project"'
,
imglib2_container_fusion
:
'
"ArrayImg"'
,
imglib2_data_container
:
'
"ArrayImg
(faster)"'
,
interpolation
:
'
"Linear
Interpolation"'
,
minimal_x
:
'
190'
,
minimal_y
:
'
-16'
,
minimal_z
:
'
-348'
,
maximal_x
:
'
1019'
,
maximal_y
:
'
1941'
,
maximal_z
:
'
486'
,
pixel_type
:
'
"16-bit
unsigned
integer"'
,
process_angle
:
'
"All
angles"'
,
process_channel
:
'
"All
channels"'
,
process_illumination
:
'
"All
illuminations"'
,
process_timepoint
:
'
"Single
Timepoint
(Select
from
List)"'
,
process_views_in_paralell
:
'
"All"'
,
xml_output
:
'
"Save
every
XML
with
user-provided
unique
id"'
}
external_transform
:
{
# channel setting: '"all_channels"'
channel_setting
:
'
"green"'
,
transform_timepoint
:
'
"All
Timepoints"'
,
transform_angle
:
'
"All
angles"'
,
transform_channel
:
'
"All
channels"'
,
# illumination setting only one illumination side
transform_illumination
:
'
"All
illuminations"'
,
apply_transformation
:
'
"Current
view
transformations
(appends
to
current
transforms)"'
,
define_mode_transform
:
'
"Matrix"'
,
matrix_transform
:
'
"0.5,
0.0,
0.0,
0.0,
0.0,
0.5,
0.0,
0.0,
0.0,
0.0,
0.5,
0.0"'
,
transformation
:
'
"Rigid"'
,
bsh_file
:
"
transform.bsh"
}
deconvolution
:
{
iterations
:
'
1'
,
minimal_x_deco
:
'
190'
,
minimal_y_deco
:
'
-16'
,
minimal_z_deco
:
'
-348'
,
maximal_x_deco
:
'
1019'
,
maximal_y_deco
:
'
1941'
,
maximal_z_deco
:
'
486'
,
detections_to_extract_psf_for_channel
:
'
"beads"'
,
process_timepoint
:
'
"Single
Timepoint
(Select
from
List)"'
,
process_angle
:
'
"All
angles"'
,
process_channel
:
'
"All
channels"'
,
process_illumination
:
'
"All
illuminations"'
,
Tikhonov_parameter
:
'
0.0006'
,
compute
:
'
"in
512x512x512
blocks"'
,
compute_on
:
'
"GPU
(Nvidia
CUDA
via
JNA)"'
,
imglib2_container
:
'
"ArrayImg"'
,
osem_acceleration
:
'
"1
(balanced)"'
,
psf_estimation
:
'
"Extract
from
beads"'
,
psf_size_x
:
'
19'
,
psf_size_y
:
'
19'
,
psf_size_z
:
'
25'
,
type_of_iteration
:
'
"Efficient
Bayesian
-
Optimization
I
(fast,
precise)"'
,
bsh_file
:
"
deconvolution_GPU.bsh"
}
hdf5_output
:
{
output_image_file_pattern
:
'
TP{{t}}_Chgreen_Ill0_Ang0,72,144,216,288.tif'
,
output_xml
:
'
"fused_Single_Channel"'
,
output_hdf5_xml
:
'
"hdf5_fused_Single_Channel"'
,
output_multiple_channels
:
'
"NO
(one
channel)"'
,
output_timepoints
:
'
0-4'
,
output_pixel_distance_x
:
0.5718
,
output_pixel_distance_y
:
0.5718
,
output_pixel_distance_z
:
0.5718
,
output_pixel_unit
:
'
um'
,
output_channels
:
"
green"
,
output_data_type
:
"
32Bit"
,
convert_32bit
:
'
"[Use
min/max
of
first
image
(might
saturate
intenities
over
time)]"'
,
output_type_of_dataset
:
'
"Image
Stacks
(ImageJ
Opener)"'
,
output_multiple_timepoints
:
'
"YES
(one
file
per
time-point)"'
,
output_multiple_angles
:
'
"NO
(one
angle)"'
,
output_illumination_directions
:
'
"NO
(one
illumination
direction)"'
,
output_imglib_container
:
'
"ArrayImg
(faster)"'
,
subsampling_output
:
'
"{{
{{1,1,1}},
{{2,2,2}},
{{4,4,4}},
{{8,8,8}}
}}"'
,
chunk_sizes_output
:
'
"{{
{{16,16,16}},
{{16,16,16}},
{{16,16,16}},
{{16,16,16}}
}}"'
,
# subsampling_output: '"{{ {{1,1,1}}, {{2,2,2}} }}"',
# chunk_sizes_output: '"{{ {{16,16,16}}, {{16,16,16}} }}"',
bsh_file_define
:
"
define_output.bsh"
,
bsh_file_hdf5
:
"
export_output.bsh"
}
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